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phcmv galv mtr  (Addgene inc)


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    Structured Review

    Addgene inc phcmv galv mtr
    Phcmv Galv Mtr, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phcmv+galv+mtr/pET28a-SENP8+(full+length))+(Plasmid+%2316361)/pmc12209767-553-26-27
    Average 93 stars, based on 10 article reviews
    phcmv galv mtr - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: BTG1 mutation yields supercompetitive B cells primed for malignant transformation
    Article Snippet: For retrovirus production (BCL2-BCL6), vectors were mixed as followed: 1 μg of pHIT60 (gag-pol), 1 μg of phCMV-GaLV MTR [a gift from D. Hodson, Addgene 163612 ( 74 )] and 4 μg of MSCV-BCL6-t2A-Bcl2 [a gift from D. Hodson, Addgene 135305 ( 74 )] and incubated for 45 min in 1 ml Opti-MEM with 18 μl of Trans-IT (Mirus Bio MIR 2300) before addition to the Lenti-X 293T dish. .. For lentivirus production (V5-tagged BTG1 WT , BTG1 Q36H , EGFP), 8.3 μg of gag-pol vector pCMVDeltaR8.91, 2.8 μg of envelop vector phCMV-GaLV MTR [a gift from D. Hodson, Addgene 163612 ( 74 )], and 11 μg of BTG1 constructs in pLEX_307 vector (described above for cell lines) were incubated for 45 min in 1 ml Opti-MEM with 33 μl of Trans-IT (Mirus Bio, MIR 2300) and added to the Lenti-X 293T dish. .. For lentivirus production (V5-tagged BTG1 WT , BTG1 Q36H , EGFP), 8.3 μg of gag-pol vector pCMVDeltaR8.91, 2.8 μg of envelop vector phCMV-GaLV MTR [a gift from D. Hodson, Addgene 163612 ( 74 )], and 11 μg of BTG1 constructs in pLEX_307 vector (described above for cell lines) were incubated for 45 min in 1 ml Opti-MEM with 33 μl of Trans-IT (Mirus Bio, MIR 2300) and added to the Lenti-X 293T dish.

    Article Title: Latent Epstein-Barr virus infection collaborates with Myc over-expression in normal human B cells to induce Burkitt-like Lymphomas in mice.
    Article Snippet: Lentiviral vectors containing shRNA targeting Myb (RHS3979201768911, RHS3979-201768912, RHS3979-201768914, RHS3979-201768915, RHS3979201786168, RHS3979-201799542) and STAT3 (RHS3979-201751956, RHS3979-201751957, RHS3979-201751958, RHS3979-201751959, RHS3979-201751960) on a pLKO backbone were obtained from Horizon Discovery (Lafayette, CO, USA). .. The following plasmids were purchased from Addgene, (Watertown, MA): pMSCV-Myc-GFP was a gift from Scott Lowe (Addgene plasmid #18770; http://n2t.net/addgene:18770; RRID:Addgene 18770), pMSCVpuro-Flag-cMyc T58A was a gift from Juan Belmonte (Addgene plasmid #20076; http://n2t.net/addgene:20076; RRID:Addgene_20076), psPAX2 was a gift from Didier Trono (Addgene plasmid #12260; http://n2t.net/addgene:12260; RRID:Addgene: 12260), phCMV-GALV-MTR was a gift from Daniel Hodson (Addgene plasmid #163612; http://n2t. net/addgene:163612; RRID:Addgene_163612), TFORF2072 (Myb expression vector) was a gift from Feng Zhang (Addgene plasmid #141905; http://n2t.net/addgene:141905; RRID: Addgene_141905), Src-Y530F was a gift from Cheng-han Yu (Addgene plasmid #124659; http://n2t.net/addgene:124659; RRID:Addgene_124659), and CA-Stat3-t2A-mCherry was a gift from Jennifer Mitchell (Addgene plasmid #127540; http://n2t.net/addgene:127540; RRID: Addgene_127540). .. The plasmid pSG5 was purchased from Stratagene. pSG5-R and pSG5-Z (kind gifts from Diane Hayward of John Hopkins University) contain the BZLF1 (Z) and BRLF1 (R) immediate-early genes driven by the SV40 promoter as previously described [76].

    Article Title: Latent Epstein-Barr virus infection collaborates with Myc over-expression in normal human B cells to induce Burkitt-like Lymphomas in mice
    Article Snippet: Lentiviral vectors containing shRNA targeting Myb (RHS3979-201768911, RHS3979-201768912, RHS3979-201768914, RHS3979-201768915, RHS3979-201786168, RHS3979-201799542) and STAT3 (RHS3979-201751956, RHS3979-201751957, RHS3979-201751958, RHS3979-201751959, RHS3979-201751960) on a pLKO backbone were obtained from Horizon Discovery (Lafayette, CO, USA). .. The following plasmids were purchased from Addgene, (Watertown, MA): pMSCV-Myc-GFP was a gift from Scott Lowe (Addgene plasmid #18770; http://n2t.net/addgene:18770 ; RRID:Addgene 18770), pMSCVpuro-Flag-cMyc T58A was a gift from Juan Belmonte (Addgene plasmid #20076; http://n2t.net/addgene:20076 ; RRID:Addgene_20076), psPAX2 was a gift from Didier Trono (Addgene plasmid #12260; http://n2t.net/addgene:12260 ; RRID:Addgene: 12260), phCMV-GALV-MTR was a gift from Daniel Hodson (Addgene plasmid #163612; http://n2t.net/addgene:163612 ; RRID:Addgene_163612), TFORF2072 (Myb expression vector) was a gift from Feng Zhang (Addgene plasmid #141905; http://n2t.net/addgene:141905 ; RRID:Addgene_141905), Src-Y530F was a gift from Cheng-han Yu (Addgene plasmid #124659; http://n2t.net/addgene:124659 ; RRID:Addgene_124659), and CA-Stat3-t2A-mCherry was a gift from Jennifer Mitchell (Addgene plasmid #127540; http://n2t.net/addgene:127540 ; RRID:Addgene_127540). .. The plasmid pSG5 was purchased from Stratagene. pSG5-R and pSG5-Z (kind gifts from Diane Hayward of John Hopkins University) contain the BZLF1 (Z) and BRLF1 (R) immediate-early genes driven by the SV40 promoter as previously described [ ].

    Article Title: Epstein-Barr virus lytic gene BNRF1 promotes B-cell lymphomagenesis via IFI27 upregulation
    Article Snippet: .. Lentiviruses for LCLs were produced by co-transfecting HEK293T cells with pCMVR8.74 (a gift from Dider Trono and Yasuo Ariumi; #22036, Addgene, Watertown, MA, USA), phCMV-GALV-MTR (a gift from Daniel Hodson; #163612, Addgene), and a third plasmid (CSII-BNRF1-HA, CSII-CMV-MCS-IRES2-Bsd, CSII-IFI27-Flag, pLV-shIFI27-T2A-mCherry, or pLV-shScramble-mCherry). .. Lentiviruses for Akata(-) cells were produced by co-transfecting HEK293T cells with pCMVR8.74, pCMV-VSV-G (a gift from Bob Weinberg; #8454, Addgene), and pLV-Tet3G or pLV-TRE-BNRF1-HA.

    Article Title: B-cell Receptor Silencing Reveals the Origin and Dependencies of High-Grade B-cell Lymphomas with MYC and BCL2 Rearrangements
    Article Snippet: IGHG1 - and CD79B -targeting gRNAs (Supplementary Table S7) were cloned into lentiCRISPRv2-Puro lentiviral vector (Addgene #98290). .. Viral particles were produced transfecting lentiCRISPRv2-Puro containing gRNAs into HEK293T packaging cell line together with psPAX2 (gift from Dr Didier Trono, Addgene plasmid # 12260) and phCMV-GALV-MTR (Addgene #163612). ..

    Construct:

    Article Title: BTG1 mutation yields supercompetitive B cells primed for malignant transformation
    Article Snippet: For retrovirus production (BCL2-BCL6), vectors were mixed as followed: 1 μg of pHIT60 (gag-pol), 1 μg of phCMV-GaLV MTR [a gift from D. Hodson, Addgene 163612 ( 74 )] and 4 μg of MSCV-BCL6-t2A-Bcl2 [a gift from D. Hodson, Addgene 135305 ( 74 )] and incubated for 45 min in 1 ml Opti-MEM with 18 μl of Trans-IT (Mirus Bio MIR 2300) before addition to the Lenti-X 293T dish. .. For lentivirus production (V5-tagged BTG1 WT , BTG1 Q36H , EGFP), 8.3 μg of gag-pol vector pCMVDeltaR8.91, 2.8 μg of envelop vector phCMV-GaLV MTR [a gift from D. Hodson, Addgene 163612 ( 74 )], and 11 μg of BTG1 constructs in pLEX_307 vector (described above for cell lines) were incubated for 45 min in 1 ml Opti-MEM with 33 μl of Trans-IT (Mirus Bio, MIR 2300) and added to the Lenti-X 293T dish. .. For lentivirus production (V5-tagged BTG1 WT , BTG1 Q36H , EGFP), 8.3 μg of gag-pol vector pCMVDeltaR8.91, 2.8 μg of envelop vector phCMV-GaLV MTR [a gift from D. Hodson, Addgene 163612 ( 74 )], and 11 μg of BTG1 constructs in pLEX_307 vector (described above for cell lines) were incubated for 45 min in 1 ml Opti-MEM with 33 μl of Trans-IT (Mirus Bio, MIR 2300) and added to the Lenti-X 293T dish.

    Article Title: Cytokine receptor IL27RA is an NF-κB–responsive gene involved in CD38 upregulation in multiple myeloma
    Article Snippet: .. For retroviral packaging vectors, see ‘Retroviral constructs’ above; for lentiviral packaging vectors, we used pCMVDeltaR8.2 (Addgene, cat. no. 12263) and phCMV-GALV-MTR (Addgene, cat. no. 163612). ..

    Incubation:

    Article Title: BTG1 mutation yields supercompetitive B cells primed for malignant transformation
    Article Snippet: For retrovirus production (BCL2-BCL6), vectors were mixed as followed: 1 μg of pHIT60 (gag-pol), 1 μg of phCMV-GaLV MTR [a gift from D. Hodson, Addgene 163612 ( 74 )] and 4 μg of MSCV-BCL6-t2A-Bcl2 [a gift from D. Hodson, Addgene 135305 ( 74 )] and incubated for 45 min in 1 ml Opti-MEM with 18 μl of Trans-IT (Mirus Bio MIR 2300) before addition to the Lenti-X 293T dish. .. For lentivirus production (V5-tagged BTG1 WT , BTG1 Q36H , EGFP), 8.3 μg of gag-pol vector pCMVDeltaR8.91, 2.8 μg of envelop vector phCMV-GaLV MTR [a gift from D. Hodson, Addgene 163612 ( 74 )], and 11 μg of BTG1 constructs in pLEX_307 vector (described above for cell lines) were incubated for 45 min in 1 ml Opti-MEM with 33 μl of Trans-IT (Mirus Bio, MIR 2300) and added to the Lenti-X 293T dish. .. For lentivirus production (V5-tagged BTG1 WT , BTG1 Q36H , EGFP), 8.3 μg of gag-pol vector pCMVDeltaR8.91, 2.8 μg of envelop vector phCMV-GaLV MTR [a gift from D. Hodson, Addgene 163612 ( 74 )], and 11 μg of BTG1 constructs in pLEX_307 vector (described above for cell lines) were incubated for 45 min in 1 ml Opti-MEM with 33 μl of Trans-IT (Mirus Bio, MIR 2300) and added to the Lenti-X 293T dish.

    Article Title: BTG1 mutation yields supercompetitive B cells primed for malignant transformation
    Article Snippet: .. For retrovirus production (BCL2-BCL6), vectors were mixed as followed: 1 μg of pHIT60 (gag-pol), 1 μg of phCMV-GaLV MTR [a gift from D. Hodson, Addgene 163612 ( 74 )] and 4 μg of MSCV-BCL6-t2A-Bcl2 [a gift from D. Hodson, Addgene 135305 ( 74 )] and incubated for 45 min in 1 ml Opti-MEM with 18 μl of Trans-IT (Mirus Bio MIR 2300) before addition to the Lenti-X 293T dish. .. For lentivirus production (V5-tagged BTG1 WT , BTG1 Q36H , EGFP), 8.3 μg of gag-pol vector pCMVDeltaR8.91, 2.8 μg of envelop vector phCMV-GaLV MTR [a gift from D. Hodson, Addgene 163612 ( 74 )], and 11 μg of BTG1 constructs in pLEX_307 vector (described above for cell lines) were incubated for 45 min in 1 ml Opti-MEM with 33 μl of Trans-IT (Mirus Bio, MIR 2300) and added to the Lenti-X 293T dish.

    Retroviral:

    Article Title: Cytokine receptor IL27RA is an NF-κB–responsive gene involved in CD38 upregulation in multiple myeloma
    Article Snippet: .. For retroviral packaging vectors, see ‘Retroviral constructs’ above; for lentiviral packaging vectors, we used pCMVDeltaR8.2 (Addgene, cat. no. 12263) and phCMV-GALV-MTR (Addgene, cat. no. 163612). ..

    Expressing:

    Article Title: Latent Epstein-Barr virus infection collaborates with Myc over-expression in normal human B cells to induce Burkitt-like Lymphomas in mice.
    Article Snippet: Lentiviral vectors containing shRNA targeting Myb (RHS3979201768911, RHS3979-201768912, RHS3979-201768914, RHS3979-201768915, RHS3979201786168, RHS3979-201799542) and STAT3 (RHS3979-201751956, RHS3979-201751957, RHS3979-201751958, RHS3979-201751959, RHS3979-201751960) on a pLKO backbone were obtained from Horizon Discovery (Lafayette, CO, USA). .. The following plasmids were purchased from Addgene, (Watertown, MA): pMSCV-Myc-GFP was a gift from Scott Lowe (Addgene plasmid #18770; http://n2t.net/addgene:18770; RRID:Addgene 18770), pMSCVpuro-Flag-cMyc T58A was a gift from Juan Belmonte (Addgene plasmid #20076; http://n2t.net/addgene:20076; RRID:Addgene_20076), psPAX2 was a gift from Didier Trono (Addgene plasmid #12260; http://n2t.net/addgene:12260; RRID:Addgene: 12260), phCMV-GALV-MTR was a gift from Daniel Hodson (Addgene plasmid #163612; http://n2t. net/addgene:163612; RRID:Addgene_163612), TFORF2072 (Myb expression vector) was a gift from Feng Zhang (Addgene plasmid #141905; http://n2t.net/addgene:141905; RRID: Addgene_141905), Src-Y530F was a gift from Cheng-han Yu (Addgene plasmid #124659; http://n2t.net/addgene:124659; RRID:Addgene_124659), and CA-Stat3-t2A-mCherry was a gift from Jennifer Mitchell (Addgene plasmid #127540; http://n2t.net/addgene:127540; RRID: Addgene_127540). .. The plasmid pSG5 was purchased from Stratagene. pSG5-R and pSG5-Z (kind gifts from Diane Hayward of John Hopkins University) contain the BZLF1 (Z) and BRLF1 (R) immediate-early genes driven by the SV40 promoter as previously described [76].

    Article Title: Latent Epstein-Barr virus infection collaborates with Myc over-expression in normal human B cells to induce Burkitt-like Lymphomas in mice
    Article Snippet: Lentiviral vectors containing shRNA targeting Myb (RHS3979-201768911, RHS3979-201768912, RHS3979-201768914, RHS3979-201768915, RHS3979-201786168, RHS3979-201799542) and STAT3 (RHS3979-201751956, RHS3979-201751957, RHS3979-201751958, RHS3979-201751959, RHS3979-201751960) on a pLKO backbone were obtained from Horizon Discovery (Lafayette, CO, USA). .. The following plasmids were purchased from Addgene, (Watertown, MA): pMSCV-Myc-GFP was a gift from Scott Lowe (Addgene plasmid #18770; http://n2t.net/addgene:18770 ; RRID:Addgene 18770), pMSCVpuro-Flag-cMyc T58A was a gift from Juan Belmonte (Addgene plasmid #20076; http://n2t.net/addgene:20076 ; RRID:Addgene_20076), psPAX2 was a gift from Didier Trono (Addgene plasmid #12260; http://n2t.net/addgene:12260 ; RRID:Addgene: 12260), phCMV-GALV-MTR was a gift from Daniel Hodson (Addgene plasmid #163612; http://n2t.net/addgene:163612 ; RRID:Addgene_163612), TFORF2072 (Myb expression vector) was a gift from Feng Zhang (Addgene plasmid #141905; http://n2t.net/addgene:141905 ; RRID:Addgene_141905), Src-Y530F was a gift from Cheng-han Yu (Addgene plasmid #124659; http://n2t.net/addgene:124659 ; RRID:Addgene_124659), and CA-Stat3-t2A-mCherry was a gift from Jennifer Mitchell (Addgene plasmid #127540; http://n2t.net/addgene:127540 ; RRID:Addgene_127540). .. The plasmid pSG5 was purchased from Stratagene. pSG5-R and pSG5-Z (kind gifts from Diane Hayward of John Hopkins University) contain the BZLF1 (Z) and BRLF1 (R) immediate-early genes driven by the SV40 promoter as previously described [ ].

    Produced:

    Article Title: Epstein-Barr virus lytic gene BNRF1 promotes B-cell lymphomagenesis via IFI27 upregulation
    Article Snippet: .. Lentiviruses for LCLs were produced by co-transfecting HEK293T cells with pCMVR8.74 (a gift from Dider Trono and Yasuo Ariumi; #22036, Addgene, Watertown, MA, USA), phCMV-GALV-MTR (a gift from Daniel Hodson; #163612, Addgene), and a third plasmid (CSII-BNRF1-HA, CSII-CMV-MCS-IRES2-Bsd, CSII-IFI27-Flag, pLV-shIFI27-T2A-mCherry, or pLV-shScramble-mCherry). .. Lentiviruses for Akata(-) cells were produced by co-transfecting HEK293T cells with pCMVR8.74, pCMV-VSV-G (a gift from Bob Weinberg; #8454, Addgene), and pLV-Tet3G or pLV-TRE-BNRF1-HA.

    Article Title: B-cell Receptor Silencing Reveals the Origin and Dependencies of High-Grade B-cell Lymphomas with MYC and BCL2 Rearrangements
    Article Snippet: IGHG1 - and CD79B -targeting gRNAs (Supplementary Table S7) were cloned into lentiCRISPRv2-Puro lentiviral vector (Addgene #98290). .. Viral particles were produced transfecting lentiCRISPRv2-Puro containing gRNAs into HEK293T packaging cell line together with psPAX2 (gift from Dr Didier Trono, Addgene plasmid # 12260) and phCMV-GALV-MTR (Addgene #163612). ..



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